Cleaning Procedure For Biological Safety Cabinet

The biological safety officer will determine the type of decontamination necessary.

Cleaning procedure for biological safety cabinet. Cleaning and disinfection 1. The biohazard bag should never be kept outside the cabinet. This may take more or less time depending on the disinfectant and the microbiological agent used. 5 1 2 dedust the biosafety cabinet daily externally with a clean dry cloth.

Hepa filters are defined as air cleaning devices that have a proven minimum removal efficiency of 99 97 of particles in the air equal to 0 3. 5 0 procedure 5 1 general cleaning procedure 5 1 1 ensure that the power supply of the biosafety cabinet is switched off. When work is completed all equipment and supplies from the bsc should be decontaminated and removed from the cabinet. It should be noted that the.

How to clean a biological safety cabinet lafw hood. 10 easy steps to cleaning a contaminated surface in the biosafety cabinet. Afterward wipe the surface with a clean dry cloth to remove the moisture. Typically surface decontamination is adequate however there are instances such as extensive repair inside the cabinet envelope or hepa filter replacement that will require gas decontamination.

Cleaning a biosafety cabinet or bsc is an important function in terms of both containment and sterility. To begin apply disinfect to a towel and decontaminate and remove items from the area of the spill. Once the cabinet has been operational for 15 minutes disinfect all interior surfaces of the cabinet thoroughly by spraying with 70 ethanol. If the biological safety cabinet is turned off between aseptic processes for routine maintenance or any other reason it should be operated long enough to allow complete purging of room air from the critical area for at least 30 minutes then cleaned and disinfected before use.

In addition periodic and thorough decontamination routines including disinfecting all removable parts and surfaces is recommended by the manufacturer and is a good laboratory practice to reduce wear on the equipment and provide greater safety to you your samples and the environment. Biological safety cabinet sop bsc 01. Spray the surfaces of all items placed into the cabinet with 70 ethanol to minimize the introduction of contaminants into the bsc. Remember to wear appropriate ppe and leave the biosafety cabinet on during spill cleanup.

Aseptic technique and daily decontamination will eliminate the majority of contaminants. 5 1 3 once in a week remove adhered dust by wet mopping using soap solution. Hepa stands for high efficiency particulate air. How to clean a biological safety cabinet lafw hood.

Put the waste from the spill into a biohazard bag inside the cabinet. Allow to air dry.